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Biological and PH Buffers

Leading Wholesaler of chaps (cas number: 75621-03-3), aces buffer (cas number: 7365-82-4), bes buffer (cas number: 10191-18-1), bicine buffer (cas number: 150-25-4), caps buffer (cas number: 1135-40-6) and hepes buffer (cas number: 7365-45-9) from Mumbai.
CHAPS (CAS Number: 75621-03-3)

CHAPS (CAS Number: 75621-03-3)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Purity>99%
ColorWhite
CAS Number75621-03-3
Physical StatePowder
Molecular Weight614.88
Empirical Formula (Hill Notation)C32H58N2O7S
Synonyms: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate, CHAPS

General description :
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.Conductivity (1.0 M, 24°C): <100 µmhos.

Application : 
CHAPS is suitable to prepare bicelles and to study the processes of liposome solubilization and reconstitution of transport proteins.
CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

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ACES Buffer (CAS Number: 7365-82-4)

ACES Buffer (CAS Number: 7365-82-4)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
ColorWhite
Physical StatePowder
Purity>99%
Molecular Weight182.20
CAS Number7365-82-4
Linear FormulaH2NCOCH2NHCH2CH2SO3H

Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)-2-aminoethanesulfonic acid, N-(Carbamoylmethyl)taurine


ACES is the common abbreviation for the compound N-(2-Acetamido)-2-aminoethanesulfonic acid.

ACES is one of Good's buffers developed in the 1960s to provide buffers with pH ranging from 6.15-8.35 for use in various applications. With a pKa of 6.9, it is often used as a buffering agent in biological and biochemical research. It is a zwitterionic buffer with a useful buffering range of 6.1-7.5. The pioneering publication by Good and his co-workers described the synthesis and physical properties of ACES buffer.

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BES Buffer (CAS Number: 10191-18-1)

BES Buffer (CAS Number: 10191-18-1)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
ColorWhite
Physical StatePowder
Purity>99%
Molecular Weight213.25
Empirical Formula (Hill Notation)C6H15NO5S
CAS Number10191-18-1

Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine


General description : 

BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.

Application : 

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.

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BICINE Buffer (CAS Number: 150-25-4)

BICINE Buffer (CAS Number: 150-25-4)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99%
Packaging TypeBottle / Drum
Molecular Weight163.17
CAS Number150-25-4
Empirical Formula (Hill Notation)C6H13NO4

Synonym: N,N-Bis(2-hydroxyethyl)glycine

Application : 

BICINE can be used to study biological buffers and zwitterionic compounds. N,N-Bis(2-hydroxyethyl)glycine has been used in a study that characterized refined crystal structures of human Ca2+/Zn2+-binding S100A3 protein. N,N-Bis(2-hydroxyethyl)glycine has also been used in a study to inform F1 antigen assembly.

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination.


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CAPS Buffer (CAS Number: 1135-40-6)

CAPS Buffer (CAS Number: 1135-40-6)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
ColorWhite
Physical StatePowder
Purity>99%
Molecular Weight221.32
Linear FormulaC6H11NH(CH2)3SO3H
CAS Number1135-40-6

Synonym: 3-(Cyclohexylamino)-1-propanesulfonic acid


Other Notes : 

Buffer for studying pH-dependent processes in enzymology; Eluent buffer component in the separation of basic drugs by HPLC

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HEPES Buffer (CAS Number:&nbsp;7365-45-9)

HEPES Buffer (CAS Number: 7365-45-9)

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Minimum Order Quantity: 1 Kg

ColorWhite
Physical StatePowder
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Purity>99.5%
Molecular Weight238.30
CAS Number7365-45-9
Empirical Formula (Hill Notation)C8H18N2O4S

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)


General description : 

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application : 

HEPES has been used:
• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• As a component of Hanks’ solution used in the degradation testing of Iron
• As a component of HEPES medium in the transfection of cell culture with plasmids
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

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HEPES Sodium Salt (CAS Number: 75277-39-3)

HEPES Sodium Salt (CAS Number: 75277-39-3)

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Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99%
Packaging TypeBottle / Drum
Molecular Weight260.29
CAS Number75277-39-3
Empirical Formula (Hill Notation)C8H17N2NaO4S

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt


General description : 

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of 6.8 to 8.2.

Application : 

HEPES sodium salt solution is suitable for cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during the phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.


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MES Buffer (CAS Number: 4432-31-9)

MES Buffer (CAS Number: 4432-31-9)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99%
Packaging TypeBottle / Drum
Molecular Weight195.24
CAS Number4432-31-9
Empirical Formula (Hill Notation)C6H13NO4S

Synonym: 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid

General description : 

MES is one of the Good′s buffers, the most extensively used biological buffers. MES is a zwitterionic N-substituted aminosulfonic acid with a morpholinic ring. It does not form complexes with majority of the metals used in environmental and biological studies. It is easily soluble in water and has minimum lipid solubility, making it impermeable to membranes.

Application : 

MES has been used as a component of the elution buffer used to elute plasma from α2-macroglobulin affinity column. It has been used as an activation buffer during antibody conjugation to microparticles. MES has been used in the functionalization of microchannels during the creation of microfluidic-integrated surface plasmon resonance (SPR) platform for pathogen detection.


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MES-H Buffer (MES Monohydrate) (CAS Number: 145224-94-8)

MES-H Buffer (MES Monohydrate) (CAS Number: 145224-94-8)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99.5%
Packaging TypeBottle / Drum
Molecular Weight213.25
CAS Number145224-94-8
Empirical Formula (Hill Notation)C6H13NO4S . H2O

Synonym: 2-(N-Morpholino)ethanesulfonic acid, 4-Morpholineethanesulfonic acid monohydrate


General description : 

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Application : 

MES Monohydrate has been used:
• To adjust the pH of growth medium to 5.5-7.0 in an in vitro cell wall stress assay
• As a buffering agent to stabilize enzymatic solutions
• As a component of PAGE running buffer

Preparation Note : 

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa =±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.

Other Notes : 

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unkown.


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MOPS Buffer (CAS Number: 1132-61-2)

MOPS Buffer (CAS Number: 1132-61-2)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99%
Product TypeBottle / Drum
Molecular Weight209.26
CAS Number1132-61-2
Empirical Formula (Hill Notation)C7H15NO4S

Synonym: 3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid


General description : 

3-(N-Morpholino)propane sulfonic acid (MOPS) is an N-substituted amino sulfonic acid with a morpholinic ring. MOPS is capable of buffering within a pH range of 6.5-7.9. MOPS is widely used in biological and biochemical studies due to its inert properties. It does not interact with any metal ions in solutions and has significant metal-buffer stability especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions. MOPS buffer maintains the pH of the mammalian cell culture medium. MOPS functions to maintain pH in denaturing gel electrophoresis of RNA. MOPS can modify lipid interactions and influence the thickness and barrier properties of membranes. MOPS interacts with bovine serum albumin and stabilizes the protein. Hydrogen peroxide oxidizes MOPS slowly to N-oxide form.

Application : 

MOPS has been used as:
• a cell culture additive component in lentiviral particle production
• as a buffering agent in microbial growth medium and nuclei extraction buffer

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PIPES Buffer (CAS Number:&nbsp;5625-37-6)

PIPES Buffer (CAS Number: 5625-37-6)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Physical StatePowder
ColorWhite
Purity>99%
Molecular Weight302.37
CAS Number5625-37-6
Empirical Formula (Hill Notation)C8H18N2O6S2

Synonym: 1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)

General description : 

PIPES is a member of the ethanesulfonic acid buffer series, developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application : 

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

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TRICINE Buffer (CAS Number: 5704-04-1)

TRICINE Buffer (CAS Number: 5704-04-1)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
ColorWhite
Packaging TypeBottle / Drum
Purity>99%
Molecular Weight179.17
CAS Number5704-04-1
Linear Formula(HOCH2)3CNHCH2CO2H

Synonym: N-[Tris(hydroxymethyl)methyl]glycine


Application : 

Buffer component for separation of low molecular weight peptides.

Tricine is a biological buffer often referred to as a “Good’s” buffer. The pKa of Tricine is 8.1 which makes Tricine is a good candidate for biological applications that require pH control above physiological. Tricine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Tricine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.

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BIS-TRIS Buffer (CAS Number: 6976-37-0)

BIS-TRIS Buffer (CAS Number: 6976-37-0)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle/ Drum
ColorWhite
Physical StatePowder
Purity>99%
Molecular Weight209.24
Empirical Formula (Hill Notation)C8H19NO5
CAS Number6976-37-0

Synonym: 2,2-Bis(hydroxymethyl)-2,2 inch,2 inch-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane

Application : 

BIS-TRIS can be used to study biological buffers. BIS-TRIS has been used in a study that synthesized ferritin grafted with temperature-responsive poly(N-isopropylacrylamide) (PIPAAm-ferritin) by a coupling reaction using PIPAAm and ferritin for obtaining stimuli-responsive biomaterials. BIS-TRIS has also been used in a study that synthesized and described the photochemical properties of oligo- ortho -azobenzenes.

Other Notes : 

Effective buffer for the assay of creatine kinase; Effectively protects hemoglobin during freeze-drying; Catholyte in IEF of 2-D gel electrophoresis.


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Tris Buffer (CAS Number: 77-86-1)

Tris Buffer (CAS Number: 77-86-1)

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Minimum Order Quantity: 1 Kg

ColorWhite
Physical StatePowder
Grade StandardUltra Pure Grade
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Purity>99.9%
Molecular Weight121.14
CAS Number77-86-1
Linear FormulaNH2C(CH2OH)3

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol


General description : 

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.

Application : 

Tris buffer was used as buffer for the following studies:
• Electrophoretic transfer for the specific identification of isozymes of starch debranching enzyme, α-amylase and 9-amylase.
• Electrophoretic separation of lipoproteins in polyacrylamide gels.
• Preparation of TRIS buffer having pH 8.
It may be used to compose DN buffer for DNA nick-end labeling of tissue sections.

Other Notes : 

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.


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Tris Hydrochloride (CAS Number: 1185-53-1)

Tris Hydrochloride (CAS Number: 1185-53-1)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Physical StatePowder
ColorWhite
Purity>99%
Packaging TypeBottle / Drum
Molecular Weight157.60
CAS Number1185-53-1
Linear FormulaNH2C(CH2OH)3 . HCl

Synonym: TRIS HCl, TRIS hydrochloride, Tris(hydroxymethyl)aminomethane hydrochloride


General description : 

Tris hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

 

Application : 

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Tris hydrochloride has been used as a buffer solution for staining procedure, reduction and alkylation studies and in situ hybridization procedure.­


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