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Biochemicals & Reagents - I to Z Listing

Wholesaler of a wide range of products which include iodonitrotetrazolium chloride (int) (cas number: 146-68-9), iptg (cas number: 367-93-1), uric acid (cas number: 69-93-2), xtt sodium (cas number: 111072-31-2), alpha-ketoglutaric acid (cas number: 328-50-7) and 1,4-bis(5-phenyloxazol-2-yl) benzene (popop) (cas number: 1806-34-4).
Iodonitrotetrazolium Chloride (INT) (CAS Number: 146-68-9)

Iodonitrotetrazolium Chloride (INT) (CAS Number: 146-68-9)

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Minimum Order Quantity: 100 Gram

Physical StatePowder
Packaging Size100 gm / 500 gm / 1 kg / 5 kg
ColorYellow
Packaging TypeBottle
CAS Number146-68-9
Molecular Weight505.70
Purity>98%
Empirical Formula (Hill Notation)C19H13ClIN5O2

Synonym: 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

INT (iodonitrotetrazolium or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium) is a commonly used tetrazolium salt (usually prepared with chloride ions), similar to tetrazolium chloride that on reduction produces a red formazan dye that can be used for quantitative redox assays. It is also toxic to prokaryotes.

INT is an artificial electron acceptor which can be utilized in a colorimetric assay to determine the concentration of protein in a solution. It can be reduced by succinate dehydrogenase to furazan, the formation of which can be measured by absorbance at 490 nm. The activity of succinate dehydrogenase is readily observed by the naked eye as the solution turns from colorless to rusty red.

Application : 

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.

 

Electron acceptor for the colorimetric assay of various dehydrogenases

Iodonitrotetrazolium chloride (INT) on reduction gets converted to iodonitrotetrazolium formazan, a water insoluble violet colored dye or indicator. Hence INT can be used for the colorimetric assay of various dehydrogenases.

Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.


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IPTG (CAS Number: 367-93-1)

IPTG (CAS Number: 367-93-1)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
CAS Number367-93-1
Purity>99%
Molecular Weight238.30
Empirical Formula (Hill Notation)C9H18O5S

Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside


Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.

Therefore, its concentration remains constant during an experiment. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into an exponentially growing bacterial culture, to give a final concentration of 1 mM. However, different concentrations of IPTG may also be used.

Application : 
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

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Uric Acid (CAS Number: 69-93-2)

Uric Acid (CAS Number: 69-93-2)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Purity>99%
Molecular Weight168.11
CAS Number69-93-2
Empirical Formula (Hill Notation)C5H4N4O3

Synonym: 2,6,8-Trihydroxypurine


Uric acid is a heterocyclic compound of carbon, nitrogen, oxygen, and hydrogen with the formula C5H4N4O3. It forms ions and salts known as urates and acid urates, such as ammonium acid urate. Uric acid is a product of the metabolic breakdown of purine nucleotides, and it is a normal component of urine. High blood concentrations of uric acid can lead to gout and are associated with other medical conditions, including diabetes and the formation of ammonium acid urate kidney stones.

Application : 

Uric acid has been used:
• in inducing hyperuricemia in mice
• as a component of digestion fluid for the simulation of in vitro human digestion model
• in selectivity test of the glucose sensor in sweat-based diabetes monitoring 

Biochem/physiol Actions : 

Uric acid is an insoluble catabolite produced by adenine and guanine metabolism. Accumulation of uric acid leads to gout, hyperuricemia, arthritis and renal failure. Elevated uric acid levels contributes to hypertension and pathogenesis of cardiovascular disease. Low uric acid levels associated with Parkinson′s disease and multiple sclerosis, may elicit protective functionality. High levels of uric acid in patients with chronic obstructive pulmonary disease (COPD) may serve as potential marker for diagnosis.

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XTT Sodium (CAS Number: 111072-31-2)

XTT Sodium (CAS Number: 111072-31-2)

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Minimum Order Quantity: 1 Gram

Physical StatePowder / Solid
Packaging TypeBottle
Packaging Size1 gm / 5 gm / 25 gm / 100 gm
ColorYellow
Molecular Weight673.52
CAS Number111072-31-2
Empirical Formula (Hill Notation)C22H16N7NaO13S2

Synonym: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt


General description : 

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.

Application : 

XTT sodium salt has been used to assess cell viability.

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines.  The tetrazolium dye has also been used to study fungal cell damage, in testing antimicrobial susceptibility of staphylococci, and in Candida biofilm research. Limitations to the XTT assay have been reported.


Biochem/physiol Actions : 

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.

Principle : 

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

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Alpha-Ketoglutaric Acid (CAS Number: 328-50-7)

Alpha-Ketoglutaric Acid (CAS Number: 328-50-7)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
ColorWhite
Packaging TypeBottle / Drum
CAS Number328-50-7
Purity>99%
Molecular Weight146.10
Linear FormulaHOOCCH2CH2COCOOH

Synonym: 2-Oxoglutaric acid, 2-Oxopentanedioic acid


α-Ketoglutaric acid (2-oxoglutaric acid) is one of two ketone derivatives of glutaric acid. The term "ketoglutaric acid," when not further qualified, almost always refers to the alpha variant. β-Ketoglutaric acid varies only by the position of the ketone functional group, and is much less common.

Its anion, α-ketoglutarate also called 2-oxoglutarate, is an important biological compound. It is the keto acid produced by deamination of glutamate, and is an intermediate in the Krebs cycle.

Application : 
α-ketoglutaric acid along with L-arginine can undergo reduction with sodium cyanoborohydride to form the diastereomers, nopaline and isonopaline.

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1,4-bis(5-Phenyloxazol-2-yl) Benzene (POPOP) (CAS Number: 1806-34-4)

1,4-bis(5-Phenyloxazol-2-yl) Benzene (POPOP) (CAS Number: 1806-34-4)

Rs 1,000  / GramGet Best Price

Minimum Order Quantity: 100 Gram

Physical StatePowder
Packaging Size100 gm / 500 gm / 1 kg / 5 kg / 10 kg
ColorLemon Yellow to Faint Green
Packaging TypeBottle
CAS Number1806-34-4
Purity>99%
Molecular Weight364.40
Empirical Formula (Hill Notation)C24H16N2O2

Synonym: 1,4-Bis(5-phenyl-2-oxazolyl)benzene, 2,2′-p-Phenylene-bis(5-phenyloxazole), Di(phenyl-5-oxazolyl-2)-1,4-benzene, POPOP


POPOP or 1,4-bis(5-phenyloxazol-2-yl) benzene is a scintillator. It is used as a wavelength shifter (also called a "secondary scintillator"), which means that it converts shorter wavelength light to longer wavelength light. Its output spectrum peaks at 410 nm, which is violet. POPOP is used in both solid and liquid organic scintillators.

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2,5-Diphenyloxazole (PPO) (CAS Number: 92-71-7)

2,5-Diphenyloxazole (PPO) (CAS Number: 92-71-7)

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Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Physical StatePowder
ColorWhite
CAS Number92-71-7
Molecular Weight221.25
Purity>99%
Empirical Formula (Hill Notation)C15H11NO

Synonym: DPO, PPO


General description : 

2,5-Diphenyloxazole (PPO) is an oxazole that can be used as a scintillator and a laser dye. It has fluorescence at a wavelength of 375 nm. It can be used as a wavelength shifter in liquid samples due to their high quantum yield and large strokes shift.

Application : 

PPO can be used as a dopant that improves the photo-electric feedback of plastic scintillators which are used as radiation detectors for nuclear materials. It can be coated on peptide substrates to facilitate radioactive labelling which can enable determination of substrate specificity of lysine methyltransferase enzymes. It may also be used in the fabrication of organic luminescent materials which can be used in light emitting diodes (LEDs).

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2,2,6,6-Tetramethylpiperidine 1-oxyl  (TEMPO) (CAS Number: 2564-83-2)

2,2,6,6-Tetramethylpiperidine 1-oxyl (TEMPO) (CAS Number: 2564-83-2)

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Minimum Order Quantity: 1 Kg

Purity>98%
Physical StatePowder
ColorOrange to Red
Packaging TypeBottle / Drum
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
CAS Number2564-83-2
Molecular Weight156.25
Empirical Formula (Hill Notation)C9H18NO

Synonym: 2,2,6,6-Tetramethyl-1-piperidinyloxy, free radical, 2,2,6,6-Tetramethylpiperidine 1-oxyl, TEMPO


General description : 

Copper/TEMPO has been utilized in the catalytic oxidation of primary alcohols to aldehydes.

 

Application : 

Catalytic oxidant for copper-catalyzed, greener oxidation of alcohols under aerobic conditions.

TEMPO (2,2,6,6-Tetramethyl-1-piperidinyloxy) was used for trapping the styrenyl radical generated from benzoyl peroxide during nitroxide-mediated radical polymerization of styrene. TEMPO-mediated oxidation was employed for the carboxylation of water-resistant nanofibrillated cellulose (NFC) films.

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Sodium Polyanethol Sulphonate (Polyanethole Sulphonic Acid,Sodium Salt) (CAS Number: 55963-78-5)

Sodium Polyanethol Sulphonate (Polyanethole Sulphonic Acid,Sodium Salt) (CAS Number: 55963-78-5)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
ColorOff White to Faint Beige Colored
CAS Number55963-78-5
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypePouch / Bottle / Drum
pH (1%)5.0-7.0

Synonym: Polyanetholesulfonic acid sodium salt


Sodium polyanetholesulfonate (SPS) is a homopolymer that was developed to be used as an anticoagulant. It is commonly used in blood culture media for bacteria because it inhibits the antimicrobial activity of immune system components present in blood. SPS inhibits the growth of some microorganisms, but this effect if often media-dependent and can be overcome by addition of gelatin or lysed blood. SPS is a weaker anticoagulant than heparin, most likely due to inhibition of antithrombin III, but a stronger inducer of platelet aggregation. It has also been used to stabilize colloidal suspensions.

Biochem/physiol Actions : 

Polyanetholesufonic acid has effect on antithrombin III activity.

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Thiazolyl Blue Tetrazolium Bromide (MTT) (CAS Number: 298-93-1)

Thiazolyl Blue Tetrazolium Bromide (MTT) (CAS Number: 298-93-1)

Rs 1,000  / GramGet Best Price

Minimum Order Quantity: 25 Gram

ColorYellow
Packaging Size25 gm / 100 gm / 500 gm / 1 kg / 5 kg
Packaging TypeBottle
Physical StatePowder
CAS Number298-93-1
Molecular Weight414.32
Purity>98%
Empirical Formula (Hill Notation)C18H16BrN5S

Synonym: 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide, MTT, Methylthiazolyldiphenyl-tetrazolium bromide


Application : 

Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Thiazolyl Blue Tetrazolium Bromide has been used for the following assays:
• Proliferation assay.
• TNF (tumor necrosis factor) bioassay.

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Thiomersal (CAS Number: 54-64-8)

Thiomersal (CAS Number: 54-64-8)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
Grade StandardUSP / BP / EP
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
ColorWhite to Pale Cream
Packaging TypeBottle
Purity>98%
Molecular Weight404.81
CAS Number54-64-8
Linear Formula2-(C2H5HgS)C6H4CO2Na

Synonym: 2-(Ethylmercuriomercapto)benzoic acid sodium salt, Ethylmercurithiosalicylic acid sodium salt, Mercury-([o-carboxyphenyl]thio)ethyl sodium salt, Sodium ethylmercurithiosalicylate


Application : 

Thimerosal is used as an antibacterial and antifungal. It is used as a topical antiseptic on skin and mucous membranes. It is also used as a preservative in pharmaceuticals as well as research.
Thiomersal is an organomercury compound that is used as an antiseptic and antifungal agent It is used as a vaccine preservative. During cell culture, thiomersal is used to prevent overgrowth in fibroblast cells. It has been used to preserve Atovaquone.

Biochem/physiol Actions : 

Thimerosal is a sulfhydryl (thiol) oxidizing reagent capable of inhibiting a wide range of sulfhydryl-dependent enzymes and proteins such as PI3-kinase-dependent methionine synthase, glutamate transport receptors, and Na++K+ dependent ATPases.

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2,3,5-Triphenyltetrazolium Chloride (TTC / Tetrazolium Salt) (CAS Number: 298-96-4)

2,3,5-Triphenyltetrazolium Chloride (TTC / Tetrazolium Salt) (CAS Number: 298-96-4)

Rs 1,000  / KgGet Best Price

Minimum Order Quantity: 1 Kg

Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
Physical StatePowder
ColorLight Yellow
Molecular Weight334.80
Purity>99%
CAS Number298-96-4
Empirical Formula (Hill Notation)C19H15ClN4

Synonym: TPTZ, TTZ, Tetrazolium Red


General description : 

2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke.

Application : 

2,3,5-Triphenyltetrazolium chloride has been used to stain heart tissue to measure the extent of acute lesion. It has also been used to stain brain tissue to check the size of infarct area.

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Triton X-100 (CAS Number: 9002-93-1)

Triton X-100 (CAS Number: 9002-93-1)

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Minimum Order Quantity: 25 Kg

Grade StandardReagent / Molecular Biology
Packaging Size25kg
Packaging TypeDrum
CAS Number9002-93-1
Usage/ApplicationLaboratory
I Deal InNew Only

Synonym: t-Octylphenoxypolyethoxyethanol, Polyethylene glycol tert-octylphenyl ether


General description : 

Triton X-100 has hemolytic property and is used for DNA extraction.

Triton X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

Application : 

Triton -X has been used:
• In the permeabilization of cells for immunofluorescence staining 
• As a component of lysis buffer in western blot analysis
• As a component of Tris-buffered saline for the preparation of cell sections in Immunogold labelling for electron microscope

Triton X-100 has been used:
• Along with Span 80 and Tween 80 in mineral oil for the preparation of oil-phase of microemulsions for PCR technique
• As a component of NX buffer which is used to pool and break down the microemulsions after PCR cycling
• In the blocking solution during immunocytochemistry procedure
• During deglycosylation procedure
• For membrane solubilization
• For cell permeabilization
• In decellularization of porcine heart valve tissue


Biochem/physiol Actions : 

Widely used non-ionic surfactant for recovery of membrane components under mild non-denaturing conditions.


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Phenylphosphate Disodium Salt Dihydrate (CAS Number: 66778-08-3)

Phenylphosphate Disodium Salt Dihydrate (CAS Number: 66778-08-3)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
CAS Number66778-08-3
Molecular Weight254.09
Linear FormulaC6H5PO4Na2 . 2H2O
Assay>98%

Synonym: Disodium phenyl phosphate dihydrate, Phenyl phosphate disodium salt


Suitable for Detection of Phosphates in milk.

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P-Nitrophenylphosphate Disodium Salt Hexahydrate (PNPP) (CAS Number: 333338-18-4)

P-Nitrophenylphosphate Disodium Salt Hexahydrate (PNPP) (CAS Number: 333338-18-4)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
ColorWhite to Pale Yellow
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
Packaging TypeBottle / Drum
CAS Number333338-18-4
Molecular Weight371.14
Purity>99%
Linear FormulaO2NC6H4OP(O)(ONa)2 . 6H2O

Synonym: p-Nitrophenyl phosphate disodium hexahydrate, Disodium 4-nitrophenyl phosphate hexahydrate, di-Sodium 4-nitrophenyl phosphate, pNPP disodium hexahydrate


Application : 

4-Nitrophenyl phosphate disodium salt hexahydrate has been used:
• as a component in diethanolamine buffer to detect alkaline phosphatase-conjugated antibodies in enzyme linked immunosorbent assay (ELISA)
• to treat adherent cells in adhesion assay
• as a phosphatase substrate in in vitro phosphatase assay in substrate buffer for ELISA
• as a substrate to measure the kinetic parameters of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) phosphatase activity
• as substrate to assay acid phosphatase activity
• as substrate to assay bound antibodies with anti-rabbit/human alkaline phosphatase conjugate

Chromogenic substrate for the determination of acid and alkaline phosphatases.  The reaction produces a soluble end product that is yellow in color. The reaction may be stopped with 3 N NaOH and read at 405 nm.


Biochem/physiol Actions : 

p-Nitrophenyl phosphate (pNPP) is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay (ELISA) procedures. It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. This product is tested for use in cell culture applications. 4-nitrophenyl phosphate (pNPP), a small-molecule substrate can be used to study on alkaline phosphatases, acid phosphatases etc.

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1,3,5-Triphenyltetrazolium Formazan (TTC Formazan) (CAS Number: 531-52-2)

1,3,5-Triphenyltetrazolium Formazan (TTC Formazan) (CAS Number: 531-52-2)

Rs 1,000  / GramGet Best Price
Physical StatePowder
Packaging Size100 gm / 500 gm / 1 kg / 5 kg / 10 kg
ColorRed to Dark Brown
Packaging TypeBottle
Purity>90%
CAS Number531-52-2
Molecular Weight300.36
Empirical Formula (Hill Notation)C19H16N4

Synonym: TTC formazan


In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains in its unreacted state in areas of necrosis since these enzymes have either denatured or degraded.

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Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) (CAS Number: 298-83-9)

Nitro Blue Tetrazolium Chloride (Nitro BT) (NBT) (CAS Number: 298-83-9)

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Minimum Order Quantity: 100 Gram

ColorYellow
Physical StatePowder
Packaging Size50 gm / 100 gm / 500 gm / 1 kg / 5 kg / 10 kg
Packaging TypeBottle
CAS Number298-83-9
Purity>99%
Molecular Weight817.64
Linear FormulaC40H30N10O6 . 2Cl
Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

General description : 

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).


Application : 

Nitrotetrazolium Blue chloride has been used:

• to determine the superoxide anion in skeletal muscle cells
• as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
• as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlings
• as a component in Triton X-100 solution to perform staining of coronal brain sections
• Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
• It has been used as a substrate for western blot detection.
• It has been used for the staining of histological tissue sections.


Biochem/physiol Actions : 

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2.

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Tetranitroblue Tetrazolium Chloride (TNBT) (CAS Number: 1184-43-6)

Tetranitroblue Tetrazolium Chloride (TNBT) (CAS Number: 1184-43-6)

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Minimum Order Quantity: 1 Gram

Packaging Size5 gm / 10 gm / 25 gm / 100 gm / 500 gm / 1 kg
Physical StatePowder
ColorWhite to Yellow
Packaging TypeBottle
CAS Number1184-43-6
Molecular Weight907.63
Empirical Formula (Hill Notation)C40H28Cl2N12O10

Synonym: 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], TNBT, Tetranitrotetrazolium blue chloride


For the cytochemical localization of Succinic Dehydrogenase redox indicator of enzyme.

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Sodium Deoxycholate (Deoxycholic Acid Sodium Salt) (CAS Number: 302-95-4)

Sodium Deoxycholate (Deoxycholic Acid Sodium Salt) (CAS Number: 302-95-4)

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Purity>98%
Physical StatePowder
ColorWhite
Packaging TypeBottle
Packaging Size1 kg / 5 kg / 10 kg / 20 kg
CAS Number302-95-4
Molecular Weight432.57
Empirical Formula (Hill Notation)C24H39NaO4 . H2O

Synonym: 3α,12α-Dihydroxy-5β-cholanic acid sodium salt, 7-Deoxycholic acid sodium salt, Desoxycholic acid sodium salt


General description : 

Sodium deoxycholate monohydrate is a lipid bile salt used in drug delivery systems.

Sodium deoxycholate (SDC) monohydrate is a bile acid salt.


Application : 

Powerful solubilization agent  ; used in the solubilization of estrogen synthetase from human placental microsomes.

Sodium deoxycholate monohydrate has been used in a study to assess a method for the immobilization of lipoxygenase in an alginate-silicate gel matrix. It has also been used in a study to investigate the in vitro dissolution of gall stones. 

 

Powerful solubilization agent; used in the solubilization of estrogen synthetase from human placental microsomes.

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Neotetrazolium Chloride (CAS Number: 298-95-3)

Neotetrazolium Chloride (CAS Number: 298-95-3)

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Minimum Order Quantity: 100 Gram

Packaging Size100 gm / 500 gm / 1 kg / 5 kg / 10 kg
CAS Number298-95-3
Packaging TypeBottle
Molecular FormulaC38H28Cl2N8
Molecular Weight667.60

Synonym: 2,2',5,5'-Tetraphenyl-3,3'-(p-diphenylene)ditetrazolium chloride, 3,3'-(4,4'-Biphenylene)bis(2,5-diphenyl-2H-tetrazolium chloride), NTC, NeoT

For Histochemical Detection of Dehydrogenase


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N,N,N,N-Tetramethyl-p-phenylenediamine Dihydrochloride (TMPPD) (CAS Number: 637-01-4)

N,N,N,N-Tetramethyl-p-phenylenediamine Dihydrochloride (TMPPD) (CAS Number: 637-01-4)

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Minimum Order Quantity: 1 Kg

Physical StatePowder
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
ColorGray
Packaging TypeBottle / Drum
Molecular Weight237.17
Purity>99%
CAS Number637-01-4
Linear FormulaC6H4[N(CH3)2]2 . 2HCl

Synonym: TMPPD, Wurster’s reagent


Application : 

Test reagent in microbiology for the classification of cytochrome oxidase positive aerobic microorganisms.; For oxidase reagent, use a 1% aqueous solution of N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride

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Sodium Pyruvate (CAS Number: 113-24-6)

Sodium Pyruvate (CAS Number: 113-24-6)

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Minimum Order Quantity: 1 Kg

Purity>99%
Physical StatePowder
ColorWhite
Packaging TypeBottle / Drum
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
CAS Number113-24-6
Molecular Weight110.04
Linear FormulaCH3COCOONa

Synonym: α-Ketopropionic acid sodium salt, 2-Oxopropanoic acid sodium salt, Pyruvic acid sodium salt


General description : 

Sodium pyruvate (SP) is a simplest keto-acid. It crystallizes as very thin plates and belongs to the monoclinic system. Its utility in Knoevenagel condensation carried out in an aqueous medium has been examined. The standard molar enthalpy of formation and molar enthalpy of dissolution of SP at infinite dilution have been obtained. Its efficacy in treating mitochondrial DNA depletion syndromes has been investigated.

Application : 

Sodium pyruvate has been used:
• To prepare Bull Tyrode′s albumin-lactate-pyruvate diluent.
• In pyruvate tolerance test.
• To prepare oligodendrocyte culture medium.

Sodium pyruvate has been used as a component:
• of cutting solution for rat brain tissue slice preparation
• of M-199 medium for the maturation of oocytes
• of Krebs-Henseleit buffer for plasma incubation

Biochem/physiol Actions : 

Sodium pyruvate based therapeutic regimen is effective for treating mitochondrial diseases. It decreases tissue damage associated with the pathophysiology of brain damage, haemorrhagic shock and ischemia-reperfusion injury models.

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Immersion Oil (CAS Number: 8000-27-9)

Immersion Oil (CAS Number: 8000-27-9)

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Minimum Order Quantity: 25 Kg

Physical StateLiquid
Packaging Size25 kg
ColorColorless to Pale Yellow
Packaging TypeBottle / Drum
GradeFor Microscopy
CAS Number8000-27-9
Synonym: Cedarwood Oil Synthetic, Immersion Oil Thickened

In light microscopy, oil immersion is a technique used to increase the resolving power of a microscope. This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index, thereby increasing the numerical aperture of the objective lens.

Immersion oils are transparent oils that have specific optical and viscosity  characteristics necessary for use in microscopy. Typical oils used have an index of refraction around 1.515. An oil immersion objective is an objective lens specially designed to be used in this way. Many condensers also give optimal resolution when the condenser lens is immersed in oil.

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Ninhydrin (CAS Number: 485-47-2)

Ninhydrin (CAS Number: 485-47-2)

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Minimum Order Quantity: 1 Kg

Purity>99%
Physical StatePowder
ColorWhite to Pale Yellow
Packaging TypeBottle / Drum
Packaging Size1 kg / 5 kg / 10 kg / 25 kg
CAS Number485-47-2
Molecular Weight178.14
Empirical Formula (Hill Notation)C9H6O4

Synonym: 1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate


Ninhydrin (2,2-dihydroxyindane-1,3-dione) is a chemical used to detect ammonia or primary and secondary amines. When reacting with these free amines, a deep blue or purple color known as Ruhemann's purple is produced. Ninhydrin is most commonly used to detect fingerprints, as the terminal amines of lysine residues in peptides and proteins sloughed off in fingerprints react with ninhydrin. It is a white solid which is soluble in ethanol and acetone at room temperature. Ninhydrin can be considered as the hydrate of indane-1,2,3-trione.

General description : 

Ninhydrin, a tricyclic 1,2,3-trione, is a useful organic building block. It is an amino acid reagent, which reveals latent fingerprints on porous surfaces like paper, cardboard and raw wood.

Application : 

Ninhydrin (Triketohydrindene hydrate) may be used as a reagent for the quantification of amino acids and peptides by photometric method and colorimetric method. It may be used to prepare the ninhydrin reagent, by mixing ninhydrin and hydrindantin (reduced form of ninhydrin) in dimethyl sulfoxide. This reagent is used in manual ninhydrin method for the quantitative determination of amino acids.

Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines. Reaction with sarcosine or proline gives azomethine ylides.

Used for the detection of free amino groups in amino acids, peptides and proteins.

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